Figure 4. Pre-incubation time with β-PMP-Glc.
The effect of pre-incubation time of cryo-solutions on post-thaw RBC integrity. (a) RBC samples were incubated for indicated time and at indicated temperature with a 110 mM β-PMP-Glc + 15% glycerol solution prior to freezing using an interrupted freezing protocol. (b) RBC samples were incubated for indicated time and at indicated temperature with a 15% glycerol solution prior to freezing using an interrupted freezing protocol. Results are presented as mean post-thaw RBC integrity (n = 4–30). Error bars represent standard error of the mean (SEM).