Skip to main content
. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Methods Mol Biol. 2015;1270:255–275. doi: 10.1007/978-1-4939-2309-0_19

Table 2.

Technique
Application SIM PALM/STORM STED
Typical Resolution (XY) 100 nm 20–50 nm 25–80 nm
Typical Resolution (Z) 250 nm 20 nm 150–600 nm
Conventional Probes Available All Some Some
Multicolor Imaging 4-color 2-color ideally 2-color ideally
Live cell imaging X Limited by sampling approach Inherently difficult
3D imaging X X X