Figure 6. HBV-induced immune cell maturation is associated with the presence of HBsAg.
(a) CD2− cells were enriched from CB using MACS beads and the presence of HBsAg was detected using immunofluorescent staining. Detection of HBsAg in CB cells (white arrow) from healthy and HBV+ mothers (green–HBsAg; blue–DAPI). Images are representative of seven CB samples per group. CB cells incubated with recombinant HBsAg was used as positive control. Scale bar, 50 μm. (b) Percentage of HBsAg+ cells among DAPI+ cells in healthy and HBV-exposed CB (n=7 per group). P-value was calculated using Mann–Whitney test. **P<0.01.