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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Neurobiol Aging. 2014 Dec 12;36(3):1274–1281. doi: 10.1016/j.neurobiolaging.2014.12.011

Fig. 3.

Fig. 3

Analyses of the cytokine production in cells from adult and aged mice on restimulation. The secretion of cytokines was measured by ELISPOT, enzyme-linked immunospot and ELISA. In A, ELISPOT assays were compared for IL-4, IFNγ, and IL-17 secreting cells in aged B6SJL mice which had received 6-peptide immunizations, the prime-boost immunizations, or 6 DNA immunizations as indicated on the x axis. The cells had been cultured in medium, Aβ42 peptide, or anti-CD3 antibody containing medium. In B, the cytokine levels for IFNγ and IL-17 were shown for these mice as measured from 72 hours cell culture supernatants. In C, IL-17 secretion was compared between adult and aged DNA Aβ42 and Aβ42 peptide immunized mice in a 48-hour ELISPOT assay (left graph) or as measured from 48 hours cell culture supernatants (right graph). Shown are mean values and SEM. Results are representative for 2 or 3 identical performed experiments. **** (p<0.0001), ns (p>0.05). Abbreviations: ELISA, enzyme-linked immunosorbent assay; SEM, standard error of the mean.