Figure 1.

SUMO2 modification of Kif18A at mitosis. (A) HeLa cells were subjected to double thymidine treatment as described in Materials & Methods. Cell pellets were lysed in 8 M urea. Equal amount of cell lysates were blotted for antibodies against Kif18A, cyclin B1, and β-actin. Kif18A blots of both short and long exposure are shown. Asterisk (*) indicates the Kif18A related signals. (B) Parental HeLa cells and HeLa cells stably expressing transfected His₆ -SUMO2 were treated with nocodazole (40 ng/mL) or taxol (40 nM) for 16 h and then lysed in 8 M urea. Equal amounts of cell lysates were used for Ni-IDA pull-down analysis. The precipitates were blotted for Kif18A and SUMO2. Kif18A-S denotes potentially SUMO-modified Kif18A.