Figure 1.

Representative photographs of immunocytochemical staining of nestin and SOX2 of human and rat NSCs. The evenly distributed neural stem cells (human) were positively stained with nestin (A, green; fluorescence) and SOX2 (D, Green; fluorescence), indicating these cells were undifferentiated human NSCs. The nuclei of cultured cells were stained by the nuclear dye, DAPI (B,E and I, blue). (C,F) are merged pictures. Similarly, rat NSCs were stained with nestin (G, green; fluorescence) and SOX2 (H, red; rhodamine), and (J) is a merged picture of (G–I). Scale bars = 60 μm.