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. 2015 Apr 8;9:115. doi: 10.3389/fnins.2015.00115

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Copyright © 2015 Liu, Mahmood, Xu, Watanabe, Biris, Hansen, Inselman, Casciano, Patterson, Paule, Slikker and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative photographs from an EdU incorporation assay from human NSCs. Both human and rat NSCs were exposed to Ag-NPs (5 μg/ml) for 24 h. The number of dividing cells was substantially decreased (B,D,F) in Ag-NP-exposed NSC cultures compared with the control (A,C,E). Quantitative analysis of the EdU-Alexa Fluor staining shows that the proliferation rate (ratio of the intensity of EdU-Alexa Fluor-stained nuclei to the intensity of DAPI-stained nuclei) of neural stem cells was significantly reduced in both human NSCs (G) and rat NSCs (H), after Ag-NP exposures. Data are presented as means ± SDs. * P < 0.05. Scale bar = 50 μm.

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