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. 2015 Apr 8;6:131. doi: 10.3389/fpls.2015.00131

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Copyright © 2015 Serrano, Kombrink and Meesters.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Estimation of assay quality by Z′ factor determination. The positive and negative controls included with a screening plate (cf. Figure 4) were used to calculate the Z′ factor, which is shown above the corresponding data set. The values of data set 1 are from a real experiment recently published (Halder and Kombrink, 2015), and the resulting Z′ factor of 0.75 indicates that this is an excellent assay for screening (quantification of GUS activity). Gradual, hypothetical increase of the negative control value (data sets 2–4) reduces the screening window and correspondingly the Z′ factor, leading to marginal (Z′ = 0.36) and unacceptable (Z′ = −0.10) assay quality for screening purposes. Likewise, increasing variability of assay data leads to decreasing Z′ factors and assay quality (data sets 5–7). Green, yellow, red indicate excellent, marginal, and inacceptable assay quality, respectively.