Figure 3.

Characterization of the purified esterase-active proteins EstA2 and EstB1. (A) Substrate specificity of various acyl chain length pNP-esters. (B) Temperature and (C) pH optimum. The assays were performed in 50 mM phosphate buffer (pH 6–7) or 50 mM Tris-HCl buffer (pH 7.2–8.8) under optimal activity parameters (80°C for EstA2 and 75°C for EstB1, respectively) against 1.25 mM pNP-butyrate. 0.065–1.3 μg of purified enzyme was used for the assays. Data represents average values and standard deviations (error bars).