Table 1.
Overview of the constructed metagenomic libraries and the esterase screening results in E. coli EPI300 and T. thermophilus BL03.
Origin of metagenomic DNA | Sample information | Number of fosmid clones | Number of lipolytic clones (60°C) | |
---|---|---|---|---|
E. coli EPI300 | T. thermophilus BL03 | |||
Hot springs (Azores Islands, Portugal) | AZ2 sediment, 60°C pH 6.0 | 384 | 1 | 1 |
AZ3 sediment, 62°C, pH 2.0 | 5472 | 0 | 4 | |
AZ4 biofilms, 60°C, pH 7.0 | 192 | 0 | 0 | |
Compost from lumber waste (Göttingen, Germany) | M12, heap II, 4th day, 63.3°C | 1920 | 1 | 1 |
Summary | 7968 | 2 | 6 |