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| Ras/effector complexes | K1, μM-1 | kon, μM-1·s-1 | k2, s-1 |
|---|---|---|---|
| RalGDS WT | 134.0 | 3.4 | 459 |
| RalGDSM26K,D47K,E54K | 5.3 | 65.0 | 347 |
| Raf WT | 9.2 | 52.0 | 480 |
RalGDS measurements were performed in a stopped-flow at 10°C, Raf at 15°C, and additional 125 mM NaCl because of the low solubility of Raf-RBD at low ionic strength.
