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. 2015 Mar 25;35(12):4830–4836. doi: 10.1523/JNEUROSCI.3571-14.2015

Figure 1.

Figure 1.

CIE of AMPARs. A, Neurons were incubated with Tf-555 and anti-GluA2 antibody for the indicated times, fixed, permeabilized, and stained for iGluA2. Arrows denote overlapping puncta visible at 20 min, but not 5 min, of internalization. n = 3. B, C, Neurons were transfected with CHC shRNA or an empty GFP vector and live labeled with Tf-555 or anti-GluA1 for 30 min 4 d after transfection. Control, Empty GFP vector. CHC shRNA blocks uptake of Tf-555 but not iGluA1. Arrows denote puncta of internalized label. n = 4. D, As above but with AP180Cmyc construct. Control, Untransfected cells. n = 3. E, Treatment with 20 μm Pitstop 2 blocks Tf-555 but not iGluA1. n = 4. Control, 0 μm Pitstop2 (DMSO). Scale bars, 10 μm.