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Loss of ScNkx2.1 early expression following cyclopamine treatment. Lateral views of the cephalic region of stage 18 catshark embryos following whole-mount in situ hybridization (ISH) with the ScNkx2.1 probe. Control (A) and cyclopamine-treated (B) embryos as described in Section “Materials and Methods” are shown. The number (n) of embryos used is indicated in each case. The signal is lost in the latter. Scale bars: 500 μm.
