Skip to main content
. 2004 Jun 14;101(25):9272–9276. doi: 10.1073/pnas.0403328101

Fig. 3.

Fig. 3.

The p110 subunit of PI3-K interacts in the nuclei with IRS-1 and UBF. (A) Lysates were made from R+ cells stimulated with IGF-I (lanes 1 and 2). Immunoprecipitation was performed with an antibody to p110, and the blot was developed with IRS-1. Lane 1, nuclear lysate; lane 2, whole-cell lysate; lane 3, lysate from 32D cells (IRS-1 negative) used as the negative control. (B) Lane 1, immunoprecipitation of nuclear lysate with an antibody to p110, the blot was developed with antibodies to UBF and IRS-1. Lanes 2 and 3, Western blots (no immunoprecipitation) of whole-cell lysates from R+ and 32D cells. Notice that UBF, but not IRS-1, is detectable in 32D cells. Lane 4, immunoprecipitation of lysates from parental 32D cells with an antibody to the p110 subunit of PI3-K.