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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: J Mol Genet Med. 2013 Aug 26;7(3):75. doi: 10.4172/1747-0862.1000075

Figure 2.

Figure 2

A typical mass-spec analysis in AR study. LNCaP cells in androgen-deprived medium were treated with DHT (dihydrotestosterone) and AR was harvested by Co-IP (Co-immunoprecipitation) for mass-spec analysis that was aligned to human AR (GenBank: M23263.1). Highlighted are identified phosphorylation corresponding to Ser94 and Ser650(GenBank: M20132.1), with Ser424 at lower frequency. Phosphorylation on Ser81 is not identified, likely due to its particular location that affects fragmentation during processing.