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. 2013 Jan 11;2:1–17. doi: 10.2147/OV.S37971

Figure 1.

Figure 1

Effects of recombinant MYXV infection of B16F10 cell cultures. (A) Cytopathic effects. Cell lines were mock-infected or infected with MYXV-Tred (moi = 0.1). Both RK13 and B16F10 cells showed significant cytopathic effects at 48 hpi. Fluorescent photomicrographs were obtained using a 560/40 nm bandpass excitation filter and an exposure time of 200 milliseconds. (B) Viral replication kinetics after inoculation of RK13 or B16F10 cells with MYXV-Tred or MYXV-IL15 (moi = 0.1). The average viral titers isolated from three experiments are shown. (C) Western blot detection of total Akt and phosphorylated Akt in MYXV-Tred-infected B16F10 cells at 0 and 4 hpi (moi = 5). (D) Average concentrations of IL-15 (pg/mL) in MYXV-IL15-infected B16F10 cell lysates (moi = 1) were detected at 1, 2, 4, and 7 dpi by enzyme-linked immunosorbent assay.

Notes: Error bars represent standard error of the mean. RK13 are rabbit kidney control cells and B16F10 is a murine melanoma cell line.

Abbreviations: dpi, days postinoculation; hpi, hours postinoculation; IL-15, interleukin-15; moi, multiplicity of infection; MYXV, myxoma virus; MYXV-IL15, MYXV coexpressing murine IL-15 and tandem dimer Tomato red fluorescent protein; MYXV-Tred, MYXV expressing tandem dimer Tomato red fluorescent protein; pfu, plaque-forming units.