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. 2013 Jan 11;2:1–17. doi: 10.2147/OV.S37971

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© 2013 Doty et al, publisher and licensee Dove Medical Press Ltd

This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

PMC Copyright notice

Lymphocyte infiltrates in and around melanoma tumors. (A) Histopathology of representative tumor sections at the indicated time points after intratumoral treatment (hematoxylin and eosin, 400×). In all treatment groups at early time points, tumors often were surrounded by dermal edema that contained mixed leukocytes (eg, MYXV-Tred and MYXV-IL15 1 dpi) and occasionally invaded the lumbar and gluteal skeletal muscle deep to the mass (eg, MYXV-IL15 2 dpi). Lymphocytes were often located surrounding vessels in the tumor (eg, MYXV-IL15 4 and 7 dpi; UV-MYXV 2 and 7 dpi; PBS 7 dpi), in the dermis (eg, MYXV-Tred 1 dpi; PBS 1 dpi), and at the junction of the tumor and the dermis (eg, MYXV-Tred 7 dpi; MYXV-IL15 survival; UV-MYXV survival; PBS 4 dpi and survival). The average grade of lymphocyte infiltrates (B) within tumors and (C) surrounding the tumors indicate an increase in lymphocyte numbers within MYXV-IL15-treated tumors 2 and 4 dpi. (D) The average combined number of CD3⁺ cells observed in the highest amounts within the tumor, at the junction of the tumor and the surrounding tissue, and within the surrounding tissue is shown.

Notes: Increased numbers of CD3⁺ cells are present within MYXV-IL15-treated tumors 2 and 4 dpi. Error bars represent standard error of the mean of three to twelve samples.

Abbreviations: CD3⁺, cluster of differentiation-3+; dpi, days postinoculation; MYXV-IL15, myxoma virus coexpressing murine interleukin-15 and tandem dimer Tomato red fluorescent protein; MYXV-Tred, myxoma virus expressing tandem dimer Tomato red fluorescent protein; PBS, phosphate-buffered saline; UV-MYXV, ultraviolet-inactivated myxoma virus.

Lymphocyte infiltrates in and around melanoma tumors. (A) Histopathology of representative tumor sections at the indicated time points after intratumoral treatment (hematoxylin and eosin, 400×). In all treatment groups at early time points, tumors often were surrounded by dermal edema that contained mixed leukocytes (eg, MYXV-Tred and MYXV-IL15 1 dpi) and occasionally invaded the lumbar and gluteal skeletal muscle deep to the mass (eg, MYXV-IL15 2 dpi). Lymphocytes were often located surrounding vessels in the tumor (eg, MYXV-IL15 4 and 7 dpi; UV-MYXV 2 and 7 dpi; PBS 7 dpi), in the dermis (eg, MYXV-Tred 1 dpi; PBS 1 dpi), and at the junction of the tumor and the dermis (eg, MYXV-Tred 7 dpi; MYXV-IL15 survival; UV-MYXV survival; PBS 4 dpi and survival). The average grade of lymphocyte infiltrates (B) within tumors and (C) surrounding the tumors indicate an increase in lymphocyte numbers within MYXV-IL15-treated tumors 2 and 4 dpi. (D) The average combined number of CD3⁺ cells observed in the highest amounts within the tumor, at the junction of the tumor and the surrounding tissue, and within the surrounding tissue is shown.

Notes: Increased numbers of CD3⁺ cells are present within MYXV-IL15-treated tumors 2 and 4 dpi. Error bars represent standard error of the mean of three to twelve samples.

Abbreviations: CD3⁺, cluster of differentiation-3+; dpi, days postinoculation; MYXV-IL15, myxoma virus coexpressing murine interleukin-15 and tandem dimer Tomato red fluorescent protein; MYXV-Tred, myxoma virus expressing tandem dimer Tomato red fluorescent protein; PBS, phosphate-buffered saline; UV-MYXV, ultraviolet-inactivated myxoma virus.