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. 2015 Mar 25;16(1):51. doi: 10.1186/s13059-015-0611-7

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© Nayfach and Pollard; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The effect of sequencing error on estimation accuracy. Unsigned error is defined as: |AĜS - AGS|/AGS. (A) MicrobeCensus was used to estimate the AGS of 20 metagenomes that were simulated with up to a 5% sequencing error rate. Metagenomes were composed of 100-bp reads from prokaryotes. (B) MicrobeCensus was used to estimate the AGS of 10 metagenomes that were composed of real Illumina reads pooled from 10 randomly chosen isolate sequencing projects.