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. 2015 Apr 8;6:278. doi: 10.3389/fmicb.2015.00278

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Copyright © 2015 Tashyreva and Elster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microcoleus vaginatus 858 CCALA before desiccation. (A–C) Culture grown under optimal conditions, viewed by light microscopy (A), stained with SYTOX Green (B), and CTC (C) fluorescent dyes; injured cells are marked with arrows. (D–F) Culture, kept at low temperature, viewed by light microscopy (D), stained with SYTOX Green (E), and CTC (F); necridic (dead) cells are SYTOX Green-positive and CTC-negative (asterisks); the injured cells are both SYTOX Green and CTC-positive (arrows). (G–I) Nitrogen-starved culture viewed by light microscopy (G), stained with SYTOX Green (H), and CTC (I); dead (in this case, decayed) cells are marked with asterisks. Scale bars are 20 μm.