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Fluorescence response of BL21DE3 cells carrying pCFR sensor plasmid and (A) pRSFMaccABCD or (B) pRSFmatCatoDA. (C,D) show two distinct fluorescence responses to IPTG induction of cells carrying pRSFmatCmatB. Na-malonate was included in the medium for (B–D). Colors indicate the concentration of IPTG used for induction: dark blue: 0.01 mM; magenta: 0.1 mM; yellow: 1 mM; cyan: 10 mM.
