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. 2015 Apr 8;3:46. doi: 10.3389/fbioe.2015.00046

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Copyright © 2015 Fehér, Libis, Carbonell and Faulon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of administering the substrate on the level of fluorescence obtained upon induction of malonyl-CoA production. Hashed bars represent the effect of IPTG on the fluorescence obtained at OD = 0.6 with constructs (A) pACYCmatCmatB, (B) pACYCmatCatoDA, and (C) pRSFmmsA. Gray bars depict the peak fluorescence detected when inducing the same constructs in the presence of their substrate: Na-malonate for matCmatB and matCatoDA and β-alanine for mmsA. The sensor plasmid was pBFR1k_RFP_8FapR for (A,B) and pCFR for (C). Asterisks indicate a significantly different fluorescence caused by the substrate (p < 0.05).