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. 2014 Oct 2;16(6):1321–1333. doi: 10.1208/s12248-014-9664-x

Fig. 3.

Fig. 3

Metabolic kinetics profiles of arctigenin (0.269–67.2 μM) in rat intestinal (RIM) and liver microsome (RLM) (n = 3) Glucuronidation in RIM: 50 mM Tris–HCl buffer (pH 7.4), 0.05 mg/ml, 2 mM UDPGA 37°C, 10 min; glucuronidation in RLM: 50 mM Tris–HCl buffer (pH 7.4), 0.2 mg/ml, 2 mM UDPGA, 37°C, 15 min; hydrolysis and demethylation in RLM: 20 mM potassium phosphate buffer (pH 7.4), 0.2 mg/ml, 2 mM NADPH, 37°C, 2 h