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. Author manuscript; available in PMC: 2015 Apr 8.
Published in final edited form as: Nat Cell Biol. 2014 Jun 29;16(8):779–791. doi: 10.1038/ncb2994

Figure 1.

Figure 1

Increased mitotic microtubule assembly rates are a common characteristic of chromosomally instable CRC cells and mediate numerical chromosome instability. a, Measurement of mitotic microtubule plus end assembly rates in various CRC cell lines expressing EB3-GFP. Scatter dot plots show average assembly rates based on measurements of 20 microtubules per cell (mean +/− SEM, t-test, n=20 cells). b, Measurement of mitotic microtubule plus end assembly rates in stable CRC cell lines expressing control or shRNAs targeting CH-TOG/CKAP5. Scatter dot plots show average assembly rates (20 microtubules/cell, mean +/− SEM, t-test, n=20 cells). c, Determination of chromosome number variability in single cell clones shown in b. The proportion of cells showing chromosome numbers deviating from the modal were determined after 30 generations (n=50–103 cells). d, Measurement of mitotic microtubule plus end assembly rates after titration of sub-nanomolar doses of Taxol®. Scatter dot plots show average assembly rates (20 microtubules/cell; mean +/− SEM, n=10 cells). e, Measurement of mitotic microtubule plus end assembly rates of single cell clones derived from CRC cell lines and grown in the absence or presence of low doses of Taxol® or after subsequent removal of Taxol®. Scatter dot plots show average assembly rates (20 microtubules/cell, mean +/− SEM, t-test, n=10 cells). f, Determination of chromosome number variability in single cell clones generated as in e. The proportion of cells showing chromosome number deviations from the modal were determined after 30 generations (n=100–109 cells). g, Measurement of mitotic microtubule plus end assembly rates in chromosomally stable HCT116 cells after treatment with low doses of nocodazole. Scatter dot plots show average assembly rates (20 microtubules/cell, mean +/− SEM, t-test, n=10 cells). h, Determination of chromosome number variability in single cell clones generated in the absence or presence of low dose nocodazole. The proportion of cells showing chromosome number deviations from the modal were determined after 30 generations (n=50 cells).

Detailed data on karyotype analyses can be found in the Supplementary Table S1. Statistic source data for Figure 1 can be found in the Supplementary Table S2.