Table 1.
The genotypes of the primary cells
CAF | NTF | |||
---|---|---|---|---|
+ | - | + | - | |
MSC requirement | CD90* | CD11B* | CD90* | CD11B* |
CD44 | CD14* | CD44 | CD14* | |
CD29* | CD19* | CD29* | CD19* | |
CD13 | CD79* | CD13 | CD79* | |
CD105* | CD117 | CD105* | CD117 | |
CD166 | CD34* | CD166 | CD34* | |
CD45* | CD45* | |||
Mesenchymal marker | ACTA2 | ACTA2 | ||
VIM | VIM | |||
Pluripotency markers | OCT4 | OCT4 | ||
SOX2 | SOX2 | |||
Reference | 18S | 18S | ||
ACTB | ACTB |
The mRNA analysis by RT-PCR of consensus markers of MSC, mesenchymal activation, and pluripotency factors. + = positive expression, − = negative expression. *The reference standard based on the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy [14].