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. 2015 Mar 11;5(3):140156. doi: 10.1098/rsob.140156

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© 2015 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 4. — Mcl-1 controls caspase-dependent DNA damage signalling and subsequent fate of cells following mitotic arrest. (a) Experimental protocol. (b) Regulation of caspase activity affects DNA damage signalling in response to mitotic arrest. Where indicated, mitotically arrested U2OS cells were co-treated with z-VAD-fmk (left panel) or Obatoclax (right panel). U2OS cells expressing exogenous Mcl-1 were compared with mock-transfected controls (middle panel). Samples were analysed by immunoblotting using the specified antibodies; asterisk denotes a non-specific signal on pS15-p53 blot. (c) Regulation of caspase activity affects cell cycle progression following a mitotic arrest. Cells were treated as indicated in (a) and analysed by flow cytometry. The cumulative histograms show the percentages of cells in the different phases of the cell cycle, according to BrdU incorporation into newly synthesized DNA and DNA content by propidium iodide staining. Percentages shown are from a representative experiment (n ≥ 3).