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Two DR-restricted CD4 determinants within peptide NY-ESO-185–102. BLCLs expressing homozygous HLA-DR molecule and a short-term T cell line (used in Fig. 2 A) and a long-term subline were used in these experiments. (A) The short-term line showed that the majority of T cells were restricted by DR2 and the minority of T cells were restricted by DR1. (B) Long-term subline derived from peptide 15-specific T cells showed exclusive DR1 restriction.
