Fig. 5.
The previously uncharacterized DR2-restricted T cells are more abundant and more polyclonal than the DP4-restricted T cells and recognize melanoma cell lines. (A) Multiple PBMC samples from patient 7 were thawed and stimulated with NY-ESO-186–99 or NY-ESO-1157–170. The T cell responses were analyzed on day 11. Note that the day-0 sample was not available for this assay. (B) An earlier analysis for NY-ESO-1157–165-specific CD8+ T cell response was performed including the day-0 sample. (C and D) T cells from day 86 after vaccination were stimulated with either NY-ESO-1157–170 or NY-ESO-186–99 and assessed with intracellular cytokine staining plus single Vβ antibodies. Vβ-positive and antigen-specific T cells were displayed as the percent of total antigen-specific T cells. (E and F) The NY-ESO-185–102-specific T cell line was used to read out antigen presentation of autologous MoDCs pulsed with NY-ESO-1 IMX (10 μg/ml, E) and DR1+ (NW-MEL-38) or DR2+ (LAR1) melanoma cell lines with or without IFN-γ treatment (F).