Table 2. Survey of DR2-restricted T cell responses among NY-ESO-1 IMX-vaccinated patients.
| NY-ESO-1/DR2 responses
|
NY-ESO-1/DP4 responses
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Patient | Anti-ESO antibody | HLA-DR2 | T cells % CD4+ prevaccination | T cells % CD4+ postvaccination | HLA-DP4 | T cells % CD4+ postvaccination | |||
| 5 | + | + | N/A | + | 0.22 | — | — | 0.03 | |
| 7 | + | + | N/A | + | 2.46 | + | + | 1.07 | |
| 9 | + | — | — | 0 | — | 0.006 | — | NT | |
| 10 | + | + | — | 0.033 | + | 0.44 | + | +/— | 0.065 |
| 13 | + | — | +/— | 0.066 | + | 0.12 | + | — | 0.017 |
| 24 | + | + | — | 0.015 | + | 0.18 | + | — | 0.018 |
| 16 | + | — | +/— | 0.068 | NT | + | +/— | 0.062 | |
| 17 | + | + | — | 0.023 | + | 0.27 | — | — | 0.01 |
| 19 | + | — | NT | — | 0.008 | + | — | 0.016 | |
| 21 | + | — | — | 0.023 | — | 0.012 | — | — | 0 |
Postvaccination samples from listed patients were tested for the DR2-restricted (NY-ESO-186—99) and DP4-restricted (NY-ESO-1157—170) responses after a 13-day culture. The positive samples were then repeated side by side with prevaccination samples, if available. Eight DR2-positive samples (six of which were also DP4+) from normal individuals were also tested for the two T cell specificities, and the results were all negative (not listed). Intracellular cytokine staining values (shown as percentage of CD4+ cells) from all negative controls were <0.03% of total gated CD4+ cells. The definite positive results are in bold. +/— potential but weak responses. NT, not tested; N/A, sample not available.