Table 2. Fragment Ions Detected by (+)NanoESI/FTICR–IRMPD MS/MS of the Quadruply Charged Precursor Ions of m/z 2076.120 Corresponding to the Ru = 15 Glycoform and Their Corresponding Assignmenta.
| m/z (exptl) | charge state | structure assignment | m/z (exptl) | charge state | structure assignment |
|---|---|---|---|---|---|
| 147.0649 | 1 | B1 | 1223.0664 | 2 | Y8 |
| 161.0805 | 1 | B1* | 1242.6708 | 1 | Z0 |
| 163.0598 | 1 | Y1/Y0 | 1260.6790 | 1 | Y0 |
| 169.0468 | 1 | B1# | 1296.1001 | 2 | Y9 |
| 185.0417 | 1 | Y1/Y0# | 1271.4889 | 2 | B17*# |
| 233.1127 | 1 | y2 | 1337.5090 | 1 | B9# |
| 239.0909 | 1 |
0,3X47/Y0/B46–H2O# or 3,5X48/Y0/B46–H2O# |
1344.5186 | 2 | B18*# |
| 257.1014 | 1 |
0,3X47/B46# or 3,5X48/Y0/B46# |
1351.5257 | 1 | B9*# |
| 275.1120 | 1 | B2–H2O | 1369.4503 | 2 | Y10 |
| 293.1225 | 1 | B2 | 1404.7199 | 1 | Z1 |
| 315.1045 | 1 | B2# | 1417.5810 | 2 | B19*# |
| 385.1485 | 1 |
0,3X47/Y0/B45-H2O# or 3,5X48/Y0/B45–H2O# |
1422.7299 | 1 | Y1 |
| 389.2136 | 1 | y3 | 1442.1545 | 2 | Y11 |
| 403.1591 | 1 |
0,3X47/Y0/B45 or 3,5X48/Y0/B45# |
1483.5663 | 1 | B10# |
| 439.1799 | 1 | B3 | 1490.5747 | 2 | B20*# |
| 461.1622 | 1 | B3# | 1497.5824 | 1 | B10*# |
| 475.1778 | 1 | B3*# | 1515.1197 | 2 | Y12 |
| 477.1365 | 1 | B3(K) | 1563.6388 | 2 | B21*# |
| 502.2979 | 1 | y4 | 1568.7865 | 1 | Y2 |
| 549.2166 | 1 |
0,3X47/Y0/B44# or 3,5X48/Y0/B44# |
1588.7016 | 2 | Y13 |
| 564.3231 | 2 | b11 | 1629.6243 | 1 | B11# |
| 585.2384 | 1 | B4 | 1636.6422 | 2 | B22*# |
| 601.366 | 1 | y5 | 1643.6409 | 1 | B11*# |
| 607.2200 | 1 | B4# | 1661.2169 | 2 | Y14 |
| 621.2357 | 1 | B4*# | 1710.1627 | 2 | B23*# |
| 630.8427 | 2 | Y0 | 1714.8474 | 1 | Y3 |
| 645.3495 | 2 | Y1/Y1 | 1775.6829 | 1 | B12# |
| 677.2633 | 1 |
0,3X47/Y0/B43–H2O# or 3,5X48/Y0/B43–H2O# |
1783.1948 | 2 | B24*# |
| 711.8683 | 2 | Y1 | 1789.6972 | 1 | B12*# |
| 716.3931 | 1 | y6 | 1856.2214 | 2 | B25*# |
| 731.2965 | 1 | B5 | 1860.9016 | 1 | Y4 |
| 753.2777 | 1 | B5# | 1921.7422 | 1 | B13# |
| 767.2934 | 1 | B5*# | 1929.2544 | 2 | B26*# |
| 784.8966 | 2 | Y2 | 1935.7594 | 1 | B13*# |
| 857.9263 | 2 | Y3 | 2002.2709 | 2 | B27*# |
| 877.3538 | 1 | B6 | 2006.9589 | 1 | Y5 |
| 899.3355 | 1 | B6# | 2067.7990 | 1 | B14# |
| 913.3508 | 1 | B6*# | 2081.8107 | 1 | B14*# |
| 930.9535 | 2 | Y4 | 2148.3278 | 2 | B29*# |
| 1003.9855 | 2 | Y5 | 2153.0157 | 1 | Y6 |
| 1045.3933 | 1 | B7# | 2213.8567 | 1 | B15# |
| 1059.4100 | 1 | B7*# | 2220.8808 | 2 | B30*# |
| 1077.0106 | 2 | Y6 | 2227.8723 | 1 | B15*# |
| 1150.0416 | 2 | Y7 | 2359.9124 | 1 | B16# |
| 1191.4511 | 1 | B8# | 2367.4345 | 2 | B32*# |
| 1205.4665 | 1 | B8*# | 2373.9205 | 1 | B16*# |
a
An asterisk
(*)
indicates fragment ions containing a methylated Rha residue; a pound sign
(#)
indicates sodiated fragment ions.