Fig. 7.

Western blot of protein-DMPO nitrone adducts (40 μg/lane, 12 % acrylamide-SDS gel) from PBMCs exposed to 100- or 10-nm silica NPs for 24 h; lane 1, 2,000 ppm (100 nm); 2, 1,000 ppm (100 nm); 3, 500 ppm (100 nm); 4, control PBMCs not exposed to NPs; 5, 1,000 ppm (10 nm); 6, 500 ppm (10 nm); 7, 250 nm (10 nm); and 8, PHA (stimulated control). The arrow indicates the nitrone antibody specifically bound to the DMPO-protein adduct via a radical derived protein oxidation following exposure to 100- or 10-nm silica NPs