Fig. 2.

Localization of RNA-binding proteins to the nucleus in the liver of torpid thirteen-lined ground squirrels (I. tridecemlineatus). a Immunostaining of endogenous TIA-1/R and PABP-1. Fluorescent signals from TIA-1/R and PABP-1 were found to be distributed in the cytoplasm and nucleus during EC but displayed distinct subnuclear foci during LT. Shown are representative cryosections immunostained with TIA-1/R and PABP-1 as well as DAPI-stained nuclei (depicted in the bottom right quadrant) from EC and LT liver samples. b Number of cells possessing subnuclear foci averaged across four fields comparing euthermic control (EC) and late torpor (LT) conditions. Data were analyzed using the Student’s t test; a denotes values are significantly different from EC, p < 0.05