Fig. 3.

Evaluation of the effects of pharmacological Hsp90 inhibition on IL-6 secretion into culture medium by HaCaT cells treated with medium alone, 2 mg/ml IgG from a healthy volunteer (normal IgG), and 2 mg/ml IgG from a bullous pemphigoid patient (BP IgG) for 6, 12, and 24 h. These BP IgG-stimulated and IgG-non-treated HaCaT cells were cultured in absence or presence of different non-toxic concentrations of 17-DMAG. IL-6 levels in cell culture supernatants were analyzed by ELISA. Results are mean ± SEM of two separate experiments, each performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001