Fig. 5.

Analysis of the impact of pharmacological Hsp90 inhibition on the activation status of NFκB in HaCaT cells treated with medium alone, 2 mg/ml IgG from a healthy volunteer (normal IgG), and 2 mg/ml IgG from a bullous pemphigoid patient (BP IgG) for 24 h. These BP IgG-stimulated and IgG-non-treated HaCaT cells were cultured in absence or presence of 1 μM 17-DMAG. NFκB p65 subunit activity in cell culture lysates was analyzed by ELISA and expressed relative to control cells incubated with medium alone. The dotted line corresponds to both cells treated with medium in absence and presence of 17-DMAG alone. Results are mean ± SEM from triplicate determinants. *P < 0.05