Fig. 3.

Survival of Artemia females and cyst development were unaffected by dsRNAs. a Representative survival of 43 females injected with GFP dsRNA (+LEA) and 36 females injected with AfrLEA1 dsRNA (−LEA) at 18 °C. b Average brood sizes produced by females injected with either GFP dsRNA (+LEA) or AfrLEA1 dsRNA (−LEA) were calculated from first, second, and third broods at 24 °C (+LEA, 53 broods; −LEA, 54 broods) and 18 °C (+LEA, 39 broods; −LEA, 32 broods). c The average time from fertilization to cyst release from females injected with either GFP dsRNA (+LEA) or AfrLEA1 dsRNA (−LEA) was determined from first, second, and third brood releases at 18 °C (+LEA, 43 broods; −LEA, 34 broods) and 24 °C (+LEA, 70 broods; −LEA, 76 broods). d day. Error bars represent standard deviation. No statistical difference was observed between +LEA and −LEA (p > 0.05) in b or c