Fig. 5.
Development of cysts after H2O2 exposure occurred independently of group 1 LEA proteins. Cysts either containing (+LEA) or lacking (−LEA) group 1 LEA proteins were incubated in sea water at room temperature for 13 to 14 weeks, exposed to H2O2, washed with sea water, and then incubated in sea water for 5 days after the last nauplius emerged. Hatched and emerged nauplii were counted. a Cysts were washed twice after H2O2 treatment. The experiment was done in triplicate for first brood cysts. +LEA: n = 40, 40, 30; 2, −LEA: n = 40, 70, 40. b Cysts were washed five times after H2O2 treatment. The experiment was done in duplicate for first brood cysts. +LEA: n = 61, 25; −LEA: n = 24, 26. c The viability (hatched + emerged nauplii) of +LEA and −LEA cysts washed either two or five times post-H2O2 treatment was determined from the samples in a and b. Error bars represent standard deviation. No statistical difference was observed between +LEA and −LEA (p > 0.05) in a, b, or c