Table 2.

Statistics of small RNA sequences from cow’s serum

Category	Heat-stress		Normal
	Sequences	Unique sequences	Sequences	Unique sequences
Raw reads	9668506	495021	13912941	545772
Low-quality reads removed	11026	3040	13931	1656
3ADT and length filtera	1202522	164358	581309	159157
mRNA, RFam, Repbase, matches removed	5005388	72864	1704071	61936
Mappable sequencesb	5278893	263210	11901654	303021

^a3ADT and length filter: reads removed due to 3ADT

^bMappable sequences: The raw reads were passed through a series of digital filters by Illumina’s Genome Analyzer Pipeline software and ACGT101-miR program, and the resulting sequences were called “mappable sequences”