Figure 1. Q6 has potent antitumor efficacy and triggers caspase-dependent apoptosis in 2 HCC cell lines. (A and B) Two HCC cell lines, HepG2 (left) and Bel-7402 (right), were treated with Q6 or TPZ (0 to 12.5 μM) for 72 h under normoxia or hypoxia. Cell viability was determined by the MTT assay. Data are representative of 3 independent experiments and are expressed as the means ± SD (C and D) HepG2 and Bel-7402 cells were treated with Q6 or TPZ (0, 10 μM) or Q6 (0 to 10 μM) +Z-VAD-FMK (40 μM) for 24 h under normoxia or hypoxia. (C) Percentage of apoptosis was measured by flow cytometry using the ANXA5/PI apoptosis detection kit. Three independent experiments were performed and the values were expressed as the mean ± SD (D) Protein levels of PARP1 were detected by western blot analysis. ACTB was measured as the loading control. Data are representative of 3 independent experiments.