Fig. 1.

Recombinant RVs expressing N fusion proteins. An RV vector, which allows the expression of an RV N fusion protein, was constructed by deleting the RV N stop codon and introducing the two single restriction sites BsiWI and NheI (BNSP-Nfu). This vector site was the target for introducing the coding sequence of GFP (BNSP-Nfu-GFP). Another RV-based vaccine vector expressing an N-GFP fusion protein from an extra gene in the RV genome was constructed by introducing the gene encoding Nfu-GFP as a new RV transcription unit in pSPBN (pSPBN-Nfu-GFP), resulting in the virus vector SPBN-Nfu-GFP. The SPBN-GFP vector schematic shows an RV vector expressing GFP alone from the same site.