Fig. 2.

Recombinant N-GFP fusion protein colocalizes with RV N. BSR cells were infected with virus vector SPBN-GFP (A and A′) or SPBN-Nfu-GFP (B and B′). Cells were fixed 2 days later and immunostained with a monoclonal antibody directed against GFP (A′ and B′) or an antibody specific for RV N protein (A and B). The results indicated that wild-type GFP has a diffuse distribution within the infected cell, whereas the Nfu-GFP fusion protein localizes similar to RV N protein.