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. 2004 Jun 10;101(25):9411–9416. doi: 10.1073/pnas.0403160101

Fig. 2.

Fig. 2.

The effect of forskolin on the DEVDase activity of cells infected with the HSV-1 d120 mutant or transduced with a baculovirus encoding gstBAD3S/A chimeric protein. (A) HEp-2 cells were either mock-infected or infected with d120 mutant (10 pfu per cell) in the presence of increasing concentrations of forskolin. Cells were harvested 18 h after infection, and the cell lysates were assayed for DEVDase activity. (B) SK-N-SH cells were either mock-infected or infected with d120 mutant (10 pfu per cell) in the presence of 100 μM forskolin. Cells were harvested 18 h after the infection and assayed for DEVDase activity. (C) Rabbit skin cells were either mock-infected or transduced with a baculovirus expressing gstBAD3S/A (10 pfu per cell), in the presence or absence of 100 μM forskolin. Cells were harvested 18 h after infection and assayed for DEVDase activity. In lanes 4 and 5, cells were exposed to either Bac-WT or Bac-US3 for 6 h, superinfected with Bac-gstBAD3S/A for 18 h, lysed, and assayed for DEVDase activity.