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. 2015 Apr 1;28(3):170–178. doi: 10.1089/vim.2014.0098

FIG. 1.

FIG. 1.

Production of hybrid immunoglobulin (Ig) A (HIgA). (A) Polymerase chain reaction (PCR) products of the variable region (lane 1), constant region (lane 2), and hybrid heavy chain (lane 3) were detected by agarose gel electrophoresis. A 100 base pairs (bp) DNA ladder was used as DNA size markers. On the left, the electrophoretic mobility of markers is shown in bp. (B) and (C) The hybrid IgA (lane 1) and IgA mAb G2G7 (lane 2) were subjected to SDS-PAGE on 7.5% gel under nonreducing conditions (B) or on 12% gel under reducing conditions (C), and visualized by Western blotting, on which the heavy chain (alpha), light chain (kappa), or J chain (J) was detected. HRP-goat antimouse IgA (1:1,000), HRP-goat antimouse kappa (1:1,500), and rabbit antimouse J chain (1:1,000) plus HRP-goat antirabbit IgG (1:5,000) were employed. G2G7 represents mouse dimeric IgA as a control. The positions of the molecular weight standards are shown on the left in kDa.