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. 2015 Apr 1;28(3):170–178. doi: 10.1089/vim.2014.0098

FIG. 3.

FIG. 3.

Production of hybrid secretory IgA (HSIgA) through in vitro association of the HIgA with SC. Partially purified dimeric HIgA and SC were incubated for 2 h at 37°C at a ratio of 5:2 (lane 1), 5:1 (lane 2), or 5:0 (lane 3) on a weight basis. In each lane, the amount of HIgA was 100 ng. The concentrations of HIgA and SC were determined by enzyme-linked immunosorbent assay (ELISA). The reassociated proteins were visualized by Western blotting after SDS-PAGE on a 7.5% gel under nonreducing (A) or reducing (B) conditions. The left panels (alpha) show the presence of heavy chains, while the right ones (pIgR) show that of SC. HRP-goat antimouse IgA (1:1,000) was used in the left panels, and goat anti-pIgR (1:1,000) plus HRP-donkey antigoat IgG (H+L; 1:3,000) in the right ones. The arrow indicates the band of HSIgA and the arrowhead the band of HIgA. The positions of molecular weight standards are shown on the left in kDa.