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. 2015 Apr 1;28(3):170–178. doi: 10.1089/vim.2014.0098

FIG. 4.

FIG. 4.

Both anti-IgA and anti-pIgR can detect the binding of HSIgA to IAV and HA. As a control, HIgA was used. (A) Binding to immobilized IAV was detected with anti-IgA by ELISA. Varying concentrations of HSIgA (filled squares) or HIgA (open circles) were allowed to bind to immobilized IAV. (B) and (C) Binding to cell surface HA was immunohistochemically detected with anti-IgA. HSIgA (B) or HIgA (C) was allowed to bind to HEK293 cells expressing HA. (D) Binding to immobilized IAV was detected with anti-pIgR by ELISA. Binding of HSIgA (filled squares) but not HIgA (open circles) was detected with anti-pIgR. (E) and (F) Binding to cell surface HA was immunohistochemically detected with anti-pIgR. Again, the binding of HSIgA (E) but not HIgA (F) to HEK293 cells expressing HA was detected with anti-pIgR. Data are expressed as means±SD in (A) and (D). The bars represent 200 μm in (B), (C), (E), and (F).