FIG. 5.
Separation of HSIgA from HIgA. (A) Chromatogram of size exclusion chromatography on TSK gel G3000SWXL. Proteins were detected at UV280 nm. The peaks containing HSIgA (fraction 13), HSIgA and HIgA (fraction 14), and free SC (arrowhead; fraction 18) are indicated. (B) Analysis of purified HSIgA by Western blotting. The number above each lane corresponds to the fraction number. Anti-IgA (left panel) or anti-pIgR (right panel) was used. SDS-PAGE was carried out on 7.5% gels under nonreducing conditions. The arrows indicate the band of HSIgA and the arrowheads the band of HIgA. The positions of molecular weight standards are shown on the left.