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IFNα is toxic to neurons cocultured with glia. Neurons were treated with a single dose of 300 IU/mL of IFNα over 12, 24, 48, and 72 h. IFNα-induced toxicity is observed at 24 h after a single dose of IFNα and persists for 72 h. No toxicity is seen at 12 h post-treatment. Graphs include data from 3 separate experiments (*P<0.05). IFNα, interferon-alpha.
