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STAT1 is phosphorylated in neurons treated with IFNα. Protein extracts from neurons treated with IFNα at various time points were analyzed by immunoblotting for STAT1 and phospho-STAT1 (p-STAT1) expression. p-STAT1 peaked at 20 min post-treatments and signal persisted for 48 h indicating IFNAR activation. Samples were run in duplicate lanes on western blot. IFNAR, IFNα receptor.
