FIG. 3.

IFNAR inhibitor blocks IFNα signaling in IFNα-treated neuronal cultures. Neurons were pretreated with saline or increasing doses of an IFNAR blocker or an IgG1 K isotype control (IFNGR) at 10 and 50 μg. Cultures were then exposed to either saline or IFNα for 20 min. JAK/STAT signaling is inhibited in cultures treated with the IFNAR blocker. ISG15 expression was found to be decreased in cultures pretreated with the IFNAR blocker compared to IFNα-alone-treated cultures (*P<0.05, **P<0.001) (A). p-STAT1 expression was decreased in cultures pretreated with the IFNAR blocker before IFNα treatment compared to cultures treated with IFNα alone STAT1, and p-STAT1 controls were run with the protein extracts as positive controls (B). Experiments were conducted in triplicates. ISG15, interferon stimulating gene 15.