Table 2. Effect of TorI on torA–lacZ fusion expression.
β-Galactosidase activity, Miller units
|
|||
---|---|---|---|
pJFi* in strains: | —IPTG | +IPTG | Ratio |
LCB620† | 250 | 35 | 7.1 |
LCB726 | 655 | 94 | 7.0 |
LCB620/pBtorR‡ | 248 | 28 | 8.9 |
Expression of torI is under the control of an IPTG-inducible promoter (ptac)
Expression of the torA—lacZ fusion was measured in the presence of TMAO
torR expression was induced with arabinose