Table 3. Effect of TorI overexpression on various plasmid-born promoters.
| β-Galactosidase activity, Miller units
|
|||
|---|---|---|---|
| pBtorI* in strains: | —ara | +ara | Ratio |
| LCB506/pPtor16† | 424 | 104 | 4.1 |
| LCB506/pPtor46 | 150 | 162 | 0.9 |
| LCB506/pPR1‡ | 20 | 18 | 1.1 |
Expression of torI is under the control of an arabinose-inducible promoter (pBAD)
Expression of the torC—lacZ fusion was measured in the presence of TMAO
torR—lacZ fusion