Table 1.
Detail of primers used for expression of developmentally important genes in hand-made cloned and IVF embryos
| Sr No | Gene | Primer sequence | Annealing temp (°C) | Size (bp) | Source | Accession No |
|---|---|---|---|---|---|---|
| 1. | GAPDH | CCTGCCAAGTATGATGAGA (F) | 53 | 131 | Bubalus bubalis | GU324291 |
| GAAGGTAGAAGAGTGAGTGT (R) | ||||||
| 2. | GLUT1 | CCGTCTCTTCCTATCCAA (F) | 56 | 194 | Bubalus bubalis | HQ434959 |
| GGTCTTCTTGAATAGTGAGTT (R) | ||||||
| 3. | OCT4 | GACAAGGAGAAGCTGGAG (F) | 54 | 75 | Bubalus bubalis | JF898834.1 |
| GCAAATTGTTCAAGGTCTTTC (R) | ||||||
| 4. | DNMT1 | ATAAGTAAGATAGTGGTTGAGTTC (F) | 55 | 100 | Bos taurus | NM_182651 |
| TTG AGC ATA CAA GGA GGA A (R) | ||||||
| 5. | BAX | ATCCACCAAGAAGCTGAG (F) | 52 | 82 | Bubalus bubalis | HE661581 |
| CTGCGATCATCCTCTGTA (R) | ||||||
| 6. | BCL2 | GGC CCCTGTTTGATTTCT (F) | 56 | 97 | Bos taurus | U92434 |
| CTTATGGCCCAGATAGGC (R) |